Methods Human umbilical vein endothelial cells ( HUVECs ) were cultured in vitro.
方法体外培养人脐静脉内皮细胞,通过传代形成细胞的衰老模型.
The cataract transgenic mice strains were established by cross breeding.
经传代培育,建立了白内障转基因小鼠鼠系.
Objective To establish human skin keratinocytes bank in vitro.
目的探索人皮肤表皮细胞分离、培养、传代、冻存及复苏技术.
Conclusion Ovary transplantation may be used for transgenic mice preservation.
结论卵巢移植可被用于转基因小鼠的传代繁殖保种.
METHODS: Upon continuously passaging, the androgen - independent LNCaP cell model was established.
方法: 通过连续传代培养,建立雄激素 非 依赖性前列腺癌细胞模型.
Immunohistochemical staining with ET ? 1 antibody showed ET ? 1 in passaged EEC was positive.
传代培养的新生小牛右心室EEC的ET-1免疫组织化学显色强阳性.
Xenotransplanted tumors and tumors after serial passage maintained the genetic stability.
异种移植瘤及其传代肿瘤保持遗传稳定性.
Keep and reproduce strain as approved SOPs.
按照批准的程序保存菌种,执行菌种传代.
After subculture, confluence was occurred in 1 - 2 weeks.
传代培养细胞可在1～2周汇合成片.
Results: Autogenous bone marrow - derived MSC showed active proliferative capacity in vitro in primary and passage cultures.
结果: 原代培养及传代培养显示,兔自体骨髓间充质干细胞具有活跃的增殖倍增能力.
We could conclude that culture and passage of donor cells might be benefit to nucleus reprogramming.
体外培养传代有利于体细胞核移植后重新编程.
The survival rates and spontaneous metastatic rates of the transplantation tumor 100 % and % , respectively.
结果:建立的间接肝原位移植瘤模型能稳定传代,移植瘤存活率100%,自发转移率达57.8%.
The bacterial contamination of the passage cell was rapidly and accurately detected with electron microscopy.
应用电镜技术检测传代细胞中的细菌污染,即快速又准确.
Methods: The primary culture implant of human umbilical arterial smooth muscle cells was adopted.
方法 选取 人脐动脉原代平滑肌细胞植块培养,得到传代细胞.
Objective To observe the effcts of IFN - α2 a on MDCK infected with influenza virus A.
目的探讨干扰素 - α2a对甲型流感病毒感染的狗肾传代细胞系(MDCK)的作用.